Journal: PLoS Pathogens

Article Title: Genome-Wide Fitness Test and Mechanism-of-Action Studies of Inhibitory Compounds in Candida albicans

doi: 10.1371/journal.ppat.0030092

Figure Lengend Snippet: Individual heterozygous deletion strains contain two unique barcodes, up-tags (red) and down-tags (blue), flanked by two pairs of common primer sequences (black for all the up-tags, grey for all the down-tags) at the deleted allele. The CaFT pool contains 2,868 strains, representing ∼45% of the C. albicans genome. Aliquots of the pool are treated with an inhibitory compound (at different concentrations) or a mock treatment over 20 population doublings. The relative abundance of each strain is subsequently monitored by DNA microarrays competitively hybridized with amplified and labeled tags (using the common primer pairs) from the two treatments. The response of each strain to the compound is appraised by a normalized z -score, with a positive value indicating hypersensitivity, and a negative value relative resistance.

Article Snippet: We custom-built a set of two DNA microarrays using Amersham CodeLink Activated Slides ( http://www.gelifesciences.com/ ).

Techniques: Amplification, Labeling